BCR-mediated apoptosis is reduced in INPP5B-depleted cells. (A) Representative histograms showing the intensities of FITC-annexin V staining of INPP5B-degron cells that were cultured in RPMI-1640 containing 2% FBS with or without anti-IgM for 6 h. (B) Apoptotic cells are expressed as a percentage of cells showing annexin V staining. Data were pooled for early (PI-negative) and late (PI-positive) apoptosis and analyzed by two-way ANOVA. (C) Protein extracts from BCR-stimulated INPP5B^{Degron/Degron} cells were blotted for NFATc1 and FOXO1 phosphorylation at the indicated time points. (D) Quantification of three such blots by densitometry. Error bars represent SEM. Data were analyzed by two-way ANOVA, and P values were calculated using Sidak multiple comparisons test. Source data are available for this figure: SourceData F7.