The defect in BCR capping in INPP5B-depleted cells is actin dependent. (A) Representative confocal images at the indicated time points from control and INPP5B-depleted cells stimulated with Texas Red–conjugated anti-IgM antibody in the presence of DMSO vs. Dyngo-4a (50 μM). Scale bar, 10 μm. Quantification of BCR capping at ∼3 min is shown on the right. Data from two independent experiments were analyzed by one-way ANOVA, and P values were calculated using Tukey’s multiple comparisons test. Error bars indicate SD. (B) Representative confocal images at the indicated time points from INPP5B-depleted cells stimulated with Texas Red–conjugated anti-IgM antibody in the presence of the indicated concentrations of LatA or DMSO. Scale bar, 10 μm. Quantification of BCR capping at ∼3 min is shown on the right. Statistical analysis was performed as above on data pooled from two independent experiments.