Figure 4.
PI(4,5)P2turnover within discrete domains of the membrane contact area is affected in INPP5B-depleted cells. (A) Representative TIRF microscopy images at the indicated time points of INPP5BDegron/Degron cells expressing a fluorescent biosensor (tubby) after stimulation on antibody-coated coverslips. PI(4,5)P2 regions of enrichment (foci) are denoted by black circles. Scale bar, 10 μm. See Videos 3 and 4 for the complete time-lapse TIRF microscopy images. (B) Statistical analysis of normalized MFI of the PI(4,5)P2 biosensor in vehicle- vs. auxin-treated INPP5BDegron/Degron cells along time. Data from three independent experiments were analyzed by two-way ANOVA, and P values were calculated using Sidak multiple comparisons test. n = 30 foci; error bars indicate SD.

PI(4,5)P 2 turnover within discrete domains of the membrane contact area is affected in INPP5B-depleted cells. (A) Representative TIRF microscopy images at the indicated time points of INPP5BDegron/Degron cells expressing a fluorescent biosensor (tubby) after stimulation on antibody-coated coverslips. PI(4,5)P2 regions of enrichment (foci) are denoted by black circles. Scale bar, 10 μm. See Videos 3 and 4 for the complete time-lapse TIRF microscopy images. (B) Statistical analysis of normalized MFI of the PI(4,5)P2 biosensor in vehicle- vs. auxin-treated INPP5BDegron/Degron cells along time. Data from three independent experiments were analyzed by two-way ANOVA, and P values were calculated using Sidak multiple comparisons test. n = 30 foci; error bars indicate SD.

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