Expression of Tsc2 in frOpn-conditioned pDCs. BM-derived pDCs from WT, Akt1^−/−, and Akt2^−/− mice were treated in vitro with frOpn1 or PBS (control) and pretreated with or without Wortmannin. Data are mean ± SEM (n = 3 wells per group) from three independent experiments. Each experimental replicate is presented with different-colored dots, and dots with black line borders are the averages derived from each replicate. ***, P ≤ 0.001 (two-way ANOVA with Bonferroni’s multiple comparison test).