A small peptide inhibitor of CaMKII substantially reduces monocyte TEM. (A) iHUVECs were transduced with CaMKIIN. 2 d after transduction, cells were lysed and their protein content resolved using SDS-PAGE and probed with Western blotting. Control (Cont) is shown for reference. GAPDH is shown as the loading control. (B) iHUVECs were fixed, permeabilized, stained for HA and VE-cadherin, and visualized using confocal immunofluorescence microscopy. Scale bar is 10 µm. (C) Monocyte transmigration across iHUVECs grown on collagen gels transduced with CaMKIIN is shown. The mouse anti-human PECAM antibody hec7 was included as a control. Data represent the average and standard deviation of three independent experiments. Each experiment comprises at least three samples with >50 leukocytes scored per sample for each condition. ** denotes P value < 0.01 with Student’s t test. MW, molecular weight.