Nanoscale arrangement of the actin regulatory proteins at the Z-disk . (A–H) Distribution of the actin-binding protein DAAM (PD and R1), Fhos, SALS, and FliI as compared with Zasp52 (A, B, D, and E), each other (C, F, and G), or Myosin S2 (H) at the Z-disk. Scale bar, 200 nm. (I) Longitudinal epitope distributions (i.e., line distances of the actin regulatory proteins relative to the Z-line), depicted as violin plots. Mean values are shown as red dots. (J) Schematic arrangement of the potential barbed-end regulatory proteins at the Z-disk. (K) Molecular model of actin regulatory protein distribution in the I-band/Z-disk region, scaled to the plots on I, with vertical lines marking the average epitope positions. F-Actin is shown in gray, DAAM in magenta, Fhos in light green, FliI in orange, SALS in red orange, Tm in green, and Cpa in cyan. Schematic transversal sections of the myofibril are shown on the left and right sides, representing the lattice structure of the thin filaments (red and green dots). Scale bar, 10 nm.