INCENP and H3T3p at KPCs and chromosome arms after ICRF-193 treatment in mitosis. (A and C) Representative immunofluorescent-stained images of pseudometaphase HeLa cells (nocodazole arrested) ± ICRF-193 treatment for 45 min. Bars, 10 µm (insets, 1 µm). (A) CREST, red; INCENP, green. (C) CREST, red. H3T3p, green. (B and D) Quantification of immunofluorescent staining at centromeres/chromosome arms. Error bars, standard deviation. Data collected from three independent experiments.