Aurora B inhibition bypasses the metaphase checkpoint induced by Topo II catalytic inhibitors. (A) Quantification of percentage preanaphase cells following nocodazole arrest and release. Cells were treated with various combinations of drugs after release from nocodazole arrest, and preanaphase cells were counted at each time point to assess mitotic progression. For each treatment and time point, n = 400 cells. Data collected from three independent experiments. Error bars, standard deviation. (B–D) Live single-cell analysis of mitotic progression. Quantitation of time to anaphase and decondensation during drug treatments. Each vertical bar represents one cell, with each cell being scored for time to anaphase and decondensation from the start of the time course. Error bars, standard deviation. Data collected from three independent experiments.