Figure 4. CENP-A and CREST colocalize regardless of ICRF-193 treatment in HeLa cells. (A) Cartoon depicts centromere/kinetochore regions. (B) Representative immunofluorescent-stained images of pseudometaphase HeLa cells (nocodazole arrested) ± ICRF-193 treatment for 45 min. CREST, red; CENP-A, green. Bars, 10 µm (insets, 1 µm). (C) Quantification of immunofluorescent staining at centromeres (Cen). Error bars, standard deviation. (D) Distribution plot of KPC-to-KPC distances. Error bars, standard deviation. Data were collected from three independent experiments.
Figure 4.

CENP-A and CREST colocalize regardless of ICRF-193 treatment in HeLa cells. (A) Cartoon depicts centromere/kinetochore regions. (B) Representative immunofluorescent-stained images of pseudometaphase HeLa cells (nocodazole arrested) ± ICRF-193 treatment for 45 min. CREST, red; CENP-A, green. Bars, 10 µm (insets, 1 µm). (C) Quantification of immunofluorescent staining at centromeres (Cen). Error bars, standard deviation. (D) Distribution plot of KPC-to-KPC distances. Error bars, standard deviation. Data were collected from three independent experiments.

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