Figure 3. Aurora B is recruited to chromosome arms in response to ICRF-193 addition in XEEs. (A and B) Mitotic replicated chromosomes were prepared from XEEs with the inhibitor treatment as in Fig. 1 A. Isolation of fixed chromosomes was performed as described in Materials and methods for immunostaining using the indicated antibodies. DNA was stained with Hoechst 33342. (C) Immunostaining of a single pair of sister chromatids showed Aurora B recruitment to chromosome arms with ICRF-193 addition. Inhibition of SUMOylation by dnUbc9 addition reduced Aurora B signals at chromosome arms. Bars, 10 µm. (D) Quantification of the mitotic chromosomes showing arm region Aurora B signals. The mitotic chromosomes showing arm region Aurora B foci were counted for >25 chromosomes from four independent experiments (n = 4). Error bars, standard deviation. *, P value from Student’s t test. *, P ≤ 0.05.
Figure 3.

Aurora B is recruited to chromosome arms in response to ICRF-193 addition in XEEs. (A and B) Mitotic replicated chromosomes were prepared from XEEs with the inhibitor treatment as in Fig. 1 A. Isolation of fixed chromosomes was performed as described in Materials and methods for immunostaining using the indicated antibodies. DNA was stained with Hoechst 33342. (C) Immunostaining of a single pair of sister chromatids showed Aurora B recruitment to chromosome arms with ICRF-193 addition. Inhibition of SUMOylation by dnUbc9 addition reduced Aurora B signals at chromosome arms. Bars, 10 µm. (D) Quantification of the mitotic chromosomes showing arm region Aurora B signals. The mitotic chromosomes showing arm region Aurora B foci were counted for >25 chromosomes from four independent experiments (n = 4). Error bars, standard deviation. *, P value from Student’s t test. *, P ≤ 0.05.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal