A screen to identify Rab GTPases that are associated with TPD54. (A) Quantification of the change in mitochondrial fluorescence intensity of GFP or GFP-Rabs 2 min after rerouting of mCherry-FKBP-TPD54 to dark MitoTrap with 200 nM rapamycin. Multiple independent experiments were completed (dots) across three independent trials. Black bars, mean ± SD. The mean ± SD for GFP (control) is also shown as a black line and gray zone, down the plot. Dunnett’s post-hoc test was done for each trial using GFP as a control. Colors indicate if P < 0.05 in one, two, or three trials, or only when all the data were pooled. n_cell = 17–36, n_exp = 3. (B) Effect size and bootstrap 95% confidence interval of the data in A. (C) The plot in B is reordered to show Rabs ranked in order of highest to lowest effect size. (D) Small multiple plots show the correlation between the mCherry-FKBP-TPD54 rerouting and GFP-Rabs co-rerouting (gray dots), a line fit to the data (black), and a y = x correlation (white).