Figure 4. TPD54 can be rerouted efficiently to mitochondria. (A) Confocal micrographs showing the rerouting of mCherry-FKBP-TPD54, but not mCherry-TPD54, to mitochondria in cells coexpressing YFP-MitoTrap after addition of 200 nM rapamycin (orange bar). (B) Kinetics of mCherry-FKBP-TPD54 rerouting. The mitochondrial and cytoplasmic signal of mCherry-FKBP-TPD54 as a function of time after the addition of 200 nM rapamycin at 10 s. Line and shaded area show the mean ± SEM, ncell = 16. (C) Still images from a TPD54 rerouting video. Time, minutes and seconds (rapamycin at 0:10). Insets, 2× zoom. Scale bars, 10 µm, 1 µm (inset).
Figure 4.

TPD54 can be rerouted efficiently to mitochondria. (A) Confocal micrographs showing the rerouting of mCherry-FKBP-TPD54, but not mCherry-TPD54, to mitochondria in cells coexpressing YFP-MitoTrap after addition of 200 nM rapamycin (orange bar). (B) Kinetics of mCherry-FKBP-TPD54 rerouting. The mitochondrial and cytoplasmic signal of mCherry-FKBP-TPD54 as a function of time after the addition of 200 nM rapamycin at 10 s. Line and shaded area show the mean ± SEM, ncell = 16. (C) Still images from a TPD54 rerouting video. Time, minutes and seconds (rapamycin at 0:10). Insets, 2× zoom. Scale bars, 10 µm, 1 µm (inset).

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