Figure 1. TPD54 is a membrane trafficking protein. (A) Representative confocal micrographs showing transiently expressed mCherry-tagged membrane trafficking proteins of interest (POI) and endogenously tagged GFP-TPD54. Inset, 3× zoom. Scale bars, 10 µm, 1 µm (inset). (B) Volcano plot of a comparative mass spectrometry analysis of GFP-TPD54 vs. GFP co-immunoprecipitation. Proteins enriched more than twofold in GFP-TPD54 samples compared with GFP are shown in red or pink; those P < 0.05 are shown in blue or pink. nexp = 4. Note, glycogen debranching enzyme (3.7-fold increase, P = 1.09 × 10−8) is not shown. Proteomic data and volcano plot calculations are available (Royle, 2019). IP, immunoprecipitation.
Figure 1.

TPD54 is a membrane trafficking protein. (A) Representative confocal micrographs showing transiently expressed mCherry-tagged membrane trafficking proteins of interest (POI) and endogenously tagged GFP-TPD54. Inset, 3× zoom. Scale bars, 10 µm, 1 µm (inset). (B) Volcano plot of a comparative mass spectrometry analysis of GFP-TPD54 vs. GFP co-immunoprecipitation. Proteins enriched more than twofold in GFP-TPD54 samples compared with GFP are shown in red or pink; those P < 0.05 are shown in blue or pink. nexp = 4. Note, glycogen debranching enzyme (3.7-fold increase, P = 1.09 × 10−8) is not shown. Proteomic data and volcano plot calculations are available (Royle, 2019). IP, immunoprecipitation.

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