Growth-based assessment of Shr3 mutant protein function. Growth of serially diluted cell suspensions from strain JKY2 (shr3Δ) carrying either vector control (VC) or the indicated plasmids expressing wild-type Shr3 (SHR3) or mutated forms of Shr3 proteins (upper case indicates alleles conferring wild-type growth; lower case indicates alleles exhibiting defective growth). The cells were spotted on YPD, SD (ammonium), and SAD (allantoin) containing toxic amino analogs as indicated at the following concentrations: 200 µg/ml metsulfuron-methyl (MM); 1 mM azetidine-2-carboxylate (AzC); 1 µg/ml L-canavanine (L-can); and 0.5% wt/vol D-histidine (D-his). Plates were photographed and growth scored after 2–3 d of incubation at 30°C. (A) Mutations SHR3-30, SHR3-31, SHR3-32, and SHR3-33. (B) Mutations SHR3-34, shr3-35, SHR3-39, and SHR3-40. (C) Mutations SHR3-41, SHR3-42, SHR3-43, and SHR3-44. (D) Mutations SHR3-45, SHR3-46, SHR3-47, and SHR3-48. (E) Mutations SHR3-49, shr3-50, SHR3-54, SHR3-55, and SHR3-56. (F) Mutations SHR3-57, SHR3-58, SHR3-59, SHR3-60, and SHR3-61. (G) Mutations SHR3-62, SHR3-63, shr3-64, SHR3-65, and SHR3-66. (H) Mutations SHR3-67, SHR3-68, SHR3-69, SHR3-70, and SHR3-83. (I) Mutations SHR3-71, SHR3-72, SHR3-73, SHR3-74, and SHR3-75. (J) Mutations shr3-76, SHR3-79, SHR3-80, SHR3-81, and shr3-82.