The importance of the individual residues affected by the shr3-35 (MSI), shr3-50 (L1), and shr3-76 (MSIV) mutations. Serial dilutions of cell suspensions from strain JKY2 (shr3Δ) carrying pRS316 (VC), pPL210 (SHR3), pAR4 (shr3-35), pPL1330 (SHR3-36), pAR47 (shr3-37), pAR37 (SHR3-38), pAR18 (shr3-50), pAR51 (SHR3-51), pAR52 (SHR3-52), pAR50 (shr3-53), pPL1349 (shr3-76), pAR48 (shr3-77), or pAR49 (SHR3-78) spotted on YPD and YPD+MM plates. Plates were incubated at 30°C for 2 d and photographed.