Figure 1.
A flow cytometric array for measuring the antibacterial reactivity of breast milk–derived IgA. (A) Design of the flow cytometric array. Made with BioRender.com. (B) Examples of Syto BC+/SSCDim staining (2 of 3,636) used to discriminate bacteria from debris/bubbles in the flow cytometer (control is empty well stained with Syto BC). Numbers represent the percentage of events inside the gate. (C) Two examples (of 101) of the magnitude of antibacterial IgA binding detected in our array comparing two donors (9 and 10) that differ in their antibacterial IgA responses. The bottom row shows the reactivity of an anti-HIV IgA antibody against the bacterial isolates (one experiment). Numbers in red represent the gMFI of that sample. (D) Breast milk–derived IgA reactivity, from 10 donors against the environmental bacteria B. japonicum. One experiment.

A flow cytometric array for measuring the antibacterial reactivity of breast milk–derived IgA. (A) Design of the flow cytometric array. Made with BioRender.com. (B) Examples of Syto BC+/SSCDim staining (2 of 3,636) used to discriminate bacteria from debris/bubbles in the flow cytometer (control is empty well stained with Syto BC). Numbers represent the percentage of events inside the gate. (C) Two examples (of 101) of the magnitude of antibacterial IgA binding detected in our array comparing two donors (9 and 10) that differ in their antibacterial IgA responses. The bottom row shows the reactivity of an anti-HIV IgA antibody against the bacterial isolates (one experiment). Numbers in red represent the gMFI of that sample. (D) Breast milk–derived IgA reactivity, from 10 donors against the environmental bacteria B. japonicum. One experiment.

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