![Response kinetics of the rod-dominated ERG are age-dependent. Rod-dominant a-wave properties of P8, P9, P10, P14, and adult retinas in response to dim flashes. (A) Representative a-wave traces normalized to their peaks illustrate an age-dependent shift in waveform kinetics. (B–D) Dim flash response properties of individual retinas (green circles; n = 1) plotted across postnatal age. After P9, tpeak (B) decreases significantly with decreasing postnatal age when compared with adult (adult: n = 14, 237 ± 16 ms; P14: n = 12, P = 0.1617 [n.s.], 177 ± 20 ms; P10: n = 12, P = 6.832 × 10−4 [***], 147 ± 17 ms; P9: n = 8, P = 4.71 × 10−5 [***], 115 ± 24 ms; P8: n = 10, P = 4.11 × 10−11 [***], 59 ± 6.8 ms). (C) When compared with adult animals, only P8 retinas displayed a longer recovery time constant (τrec; see Eq. 3) compared with all other ages (adult: n = 14, 269 ± 31 ms; P14: n = 12, P = 0.999 [n.s.], 183 ± 20 ms; P10: n = 12, P = 0.999 [n.s.], 206 ± 26 ms; P9: n = 8, P = 0.999 [n.s.], 271 ± 14 ms; P8: n = 10, P = 0.0163 [*], 751 ± 145 ms). (D) Conversely, integration time (tint) was exclusively extended at maturity compared to all other ages (adult: n = 14, 269 ± 31 ms; P14: n = 12, P = 0.00011, 183 ± 20 ms; P10: n = 12, P = 0.0015, 206 ± 26 ms; P9: n = 8, P = 1.3407 × 10−5, 271 ± 43 ms; P8: n = 10, P = 3.1630 × 10−5, 195 ± 15 ms). Black bars represent the population means. Single, double, and triple asterisks indicate a P value of <0.05, <0.01, and <0.001, respectively.](https://cdn.rupress.org/rup/content_public/journal/jgp/155/9/10.1085_jgp.202213262/1/jgp_202213262_fig4.png?Expires=1767665909&Signature=2Z6nHQI7rsG-9-~TF1f363NfIYIH2wDOtBbZ4lm0CzOi0E7uYCpGvRk6yu2lE~1f77-JxUJePl~o6Si0N41v86XbnRVyXdXaenXo1Xxt9zj~oOdlCEh08sA3Sr0fuDxgGeJF8OQ3vyQtksWO8n8ZPVc67EUjsO7G4lbDFFG~5AEdIZrfenLE7esYSp2m4M350NgkeiasOYZucE6RysiVD3e7sy7zepzhHVkAZaxhz71mVmG3JdApqqw3WYcCMLN44NGkxbtrH49urjHcW5~feYnno7H4aRi-GVN4YOwMo2jijJCfnlQaAE-0OO4SGoXVVpyylhFkF3AIA7bK2pnbGA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Response kinetics of the rod-dominated ERG are age-dependent. Rod-dominant a-wave properties of P8, P9, P10, P14, and adult retinas in response to dim flashes. (A) Representative a-wave traces normalized to their peaks illustrate an age-dependent shift in waveform kinetics. (B–D) Dim flash response properties of individual retinas (green circles; n = 1) plotted across postnatal age. After P9, tpeak (B) decreases significantly with decreasing postnatal age when compared with adult (adult: n = 14, 237 ± 16 ms; P14: n = 12, P = 0.1617 [n.s.], 177 ± 20 ms; P10: n = 12, P = 6.832 × 10−4 [***], 147 ± 17 ms; P9: n = 8, P = 4.71 × 10−5 [***], 115 ± 24 ms; P8: n = 10, P = 4.11 × 10−11 [***], 59 ± 6.8 ms). (C) When compared with adult animals, only P8 retinas displayed a longer recovery time constant (τrec; see Eq. 3) compared with all other ages (adult: n = 14, 269 ± 31 ms; P14: n = 12, P = 0.999 [n.s.], 183 ± 20 ms; P10: n = 12, P = 0.999 [n.s.], 206 ± 26 ms; P9: n = 8, P = 0.999 [n.s.], 271 ± 14 ms; P8: n = 10, P = 0.0163 [*], 751 ± 145 ms). (D) Conversely, integration time (tint) was exclusively extended at maturity compared to all other ages (adult: n = 14, 269 ± 31 ms; P14: n = 12, P = 0.00011, 183 ± 20 ms; P10: n = 12, P = 0.0015, 206 ± 26 ms; P9: n = 8, P = 1.3407 × 10−5, 271 ± 43 ms; P8: n = 10, P = 3.1630 × 10−5, 195 ± 15 ms). Black bars represent the population means. Single, double, and triple asterisks indicate a P value of <0.05, <0.01, and <0.001, respectively.