Figure S2. Reduction of DC and monocyte... | Rockefeller University Press

Figure S2.

$Reduction of DC and monocyte progenitors in BM by EL4-IL-6. (A) Lin− BM from Zbtb46egfp/+ mice was analyzed by FACS. cKitintZbtb46-EGFP+ cells were examined for MHCII expression. Numbers represent the percentage of cells in the indicated gate. (B)Zbtb46egfp/+ mice were inoculated i.p. with PBS, EL4-empty, or EL4-IL-6 (106 cells) as described in Fig. 2. BM cells were analyzed by FACS on day 14 after inoculation. Shown are pre-cDC1 progenitors defined as gate R1 in Fig. 2 A (Grajales-Reyes et al., 2015). (C) Bar-scatter graphs for absolute number of pre-cDC1 from Fig. 2, F and G (average # ± SD). Cells were enumerated from total BM cells collected from two femurs and tibias. Numbers of mice for each experimental group are indicated as dots. (D)Zbtb46egfp/+ mice injected i.p. with PBS, EL4-empty, or EL4-IL-6 (106 cells), and the BM progenitors were analyzed on day 14 after inoculation. Shown are analyses for MDP (cKithi Flt3+ M-CSFR+; red gate), CDP (cKitint Flt3+ M-CSFR+ MHCII− CD11c−; blue gate) and monocyte progenitors (cKit+ Flt3− M-CSFR+), mostly cMoPs (black gate). Data shown are representative of two independent experiments. (E) Bar-scatter graphs show the frequency of MDP (red), CDP (blue), and monocyte progenitors (cKithi, gray bars; ckitint, white bars) as a fraction of Lin− BM cells for the mice in D (average % ± SD). Each dot represents an individual mouse for PBS (n = 7), EL4-empty (n = 9), EL4-IL-6 (n = 11). *P < 0.05, **P < 0.01, ****P < 0.0001 (Student’s t test). Refer to the image caption for details.$

Reduction of DC and monocyte progenitors in BM by EL4-IL-6. (A) Lin⁻ BM from Zbtb46^egfp/+ mice was analyzed by FACS. cKit^intZbtb46-EGFP⁺ cells were examined for MHCII expression. Numbers represent the percentage of cells in the indicated gate. (B)Zbtb46^egfp/+ mice were inoculated i.p. with PBS, EL4-empty, or EL4-IL-6 (10⁶ cells) as described in Fig. 2. BM cells were analyzed by FACS on day 14 after inoculation. Shown are pre-cDC1 progenitors defined as gate R1 in Fig. 2 A (Grajales-Reyes et al., 2015). (C) Bar-scatter graphs for absolute number of pre-cDC1 from Fig. 2, F and G (average # ± SD). Cells were enumerated from total BM cells collected from two femurs and tibias. Numbers of mice for each experimental group are indicated as dots. (D)Zbtb46^egfp/+ mice injected i.p. with PBS, EL4-empty, or EL4-IL-6 (10⁶ cells), and the BM progenitors were analyzed on day 14 after inoculation. Shown are analyses for MDP (cKit^hi Flt3⁺ M-CSFR⁺; red gate), CDP (cKit^int Flt3⁺ M-CSFR⁺ MHCII⁻ CD11c⁻; blue gate) and monocyte progenitors (cKit⁺ Flt3⁻ M-CSFR⁺), mostly cMoPs (black gate). Data shown are representative of two independent experiments. (E) Bar-scatter graphs show the frequency of MDP (red), CDP (blue), and monocyte progenitors (cKit^hi, gray bars; ckit^int, white bars) as a fraction of Lin⁻ BM cells for the mice in D (average % ± SD). Each dot represents an individual mouse for PBS (n = 7), EL4-empty (n = 9), EL4-IL-6 (n = 11). *P < 0.05, **P < 0.01, ****P < 0.0001 (Student’s t test).