Lattice structure of permeabilized fresh and frozen porcine myocardium. (A and B) Representative x-ray diffraction patterns from permeabilized fresh (A) and frozen (B) porcine myocardium. (C) The equatorial intensity ratio (I1,1/I1,0) from permeabilized fresh (Fre) and frozen (Fro) porcine myocardium. (D) The lattice spacing (d1,0) from permeabilized fresh and frozen porcine myocardium. (E and F) The EM cross-sections are views of sarcomere A-bands from permeabilized fresh (E) and frozen (F) porcine myocardium. The thick filaments (yellow circles) are surrounded by six hexagonally arranged thin filaments (red hexagons). (G and H) σd (G) and σs (H) in fresh and frozen myocardium. ns: P ≥ 0.05, and ****: P < 0.0001. Solid and open symbols are from different biological repeats. The error bars in the graphs are SEM.