PIG-T interacts with CLPTM1L and is itself involved in MICA*008 maturation and surface expression. (A and B) Co-IP performed in RKO*008 CLPTM1L KO cells expressing an EV (KO EV) or a C’FLAG tagged CLPTM1L (Rescue C’FLAG). Detection Abs are indicated next to each blot; ND, not detected. (A) Cell lysates before precipitation were used as input control. (B) Precipitation Ab used was α-FLAG. (C) 293T cells, either parental (293T) or after PIG-T KO, were lysed and analyzed by WB. Detection Abs are indicated on the left. Arrow shows a specific PIG-T band (~65 kD). (D) Cells as in C were stained with the indicated Abs and analyzed by FCM. 293T, black line, PIG-T KO, gray line. Background (secondary Ab only), gray-filled histogram. (E and F) Co-IP performed in RKO*008 cells, co-expressing either EV or US9-HIS (US9). Detection Abs are indicated next to each blot. ND, not detected. (E) Cell lysates before precipitation were used as input control. (F) Precipitation Ab used was α-PIG-K. Asterisk indicates the specific PIG-K band (~42 kD). Source data are available for this figure: SourceData F7.