PIKfyve activity is required for its dissociation from phagosomes. (A) Timelapses of PIKfyve-GFP expressing cells during phagocytosis of pHrodo-yeast (red) before (top) and after (bottom) addition of 5 μM apilimod. (B) The PIKfyve-GFP intensity around the phagosome in these movies. (C) PIKfyve-GFP dynamics over multiple events by manually scoring the time of PIKfyve-GFP delivery and release. 14 untreated events and 10 apilimod-treated events were scored over three experiments, each with the thick line indicating the proportion of GFP-positive phagosomes at each time after engulfment. Thin lines represent individual events. All scale bars = 2 μm.