Expression levels of microinjected CENP-O–GFP are equal between constructs. (A and B) Quantification of CENP-O–GFP levels in cytoplasm for constructs analyzed in Fig. 6, A and B (A) and Fig. 6, C and D (B). Each spot represents one cell. For each construct, 38 cytoplasmic ROIs per cell were analyzed (see Materials and methods for details) from ≥19 cells from three (A) or two (B) independent experiments. Bars: mean intensity with standard deviation. *P < 0.05; ns: not significant; one-way ANOVA with Tukey’s multiple comparison test (A) or two-tailed Student's T test (B).