Signatures of positive selection in loops and turns of rodent and primate CENP-O C-terminal RWD domains. (A and B) Most likely adaptive residues in rodent (A) or primate (B) CENP-O mapped to the mouse or human CENP-O coding sequence, respectively, by FREEDA. Blue frames were manually added to outline C-terminal RWD domains. (C and D) FREEDA-annotated C-terminal RWD domains of mouse (C) and human (D) CENP-O. Magenta: highly likely adaptive residues (probability ≥ 0.9).