Signatures of positive selection in the CENP-P C-terminal RWD domain. (A) Annotated structural prediction model of MmCENP-P, generated automatically by FREEDA and visualized in PyMOL without manual modifications. The C-terminal RWD domain (labeled by the user within the GUI) is in gray. (B) Three residues (magenta) with the highest probability of having evolved under positive selection (≥0.9) are shown in snippets of the multiple sequence alignment in Murinae. Dark gray: highly conserved residues, gray: less conserved residues, white: non-synonymous substitutions.