Comparison of mNG-tagged UNC-94 isoforms and covisualization with CAP-1 and actin filaments. (A) Schematic of the endogenous unc-94 locus fused to mNG at its C-terminus (tagging both isoforms) or at the N-termini of the isoforms encoding either UNC-94A or UNC-94B. (B) Micrographs depicting expression and localization of UNC-94A::mNG, UNC-94B::mNG, and UNC-94::mNG from a ventral view. (C) Bar plot depicting relative intensity measurements of whole embryos with the UNC-94A::mNG, UNC-94B::mNG, and UNC-94::mNG alleles (n ≥ 5 embryos). (D) Representative micrograph of UNC-94::mNG using imaging parameters optimized for static imaging. (E) Micrographs depicting localization of mex-5p::LifeAct::mScarlet-I, mTurquoise2::CAP-1, and UNC-94::mNG. Ea and Ep are indicated with yellow dotted lines or arrowheads. Error bars indicate SD. Scale bars: 5 µm.
Figure S3.

Comparison of mNG-tagged UNC-94 isoforms and covisualization with CAP-1 and actin filaments. (A) Schematic of the endogenous unc-94 locus fused to mNG at its C-terminus (tagging both isoforms) or at the N-termini of the isoforms encoding either UNC-94A or UNC-94B. (B) Micrographs depicting expression and localization of UNC-94A::mNG, UNC-94B::mNG, and UNC-94::mNG from a ventral view. (C) Bar plot depicting relative intensity measurements of whole embryos with the UNC-94A::mNG, UNC-94B::mNG, and UNC-94::mNG alleles (n ≥ 5 embryos). (D) Representative micrograph of UNC-94::mNG using imaging parameters optimized for static imaging. (E) Micrographs depicting localization of mex-5p::LifeAct::mScarlet-I, mTurquoise2::CAP-1, and UNC-94::mNG. Ea and Ep are indicated with yellow dotted lines or arrowheads. Error bars indicate SD. Scale bars: 5 µm.

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