Localization of barbed- and pointed-end capping proteins CAP-1 and UNC-94. (A) Schematic of the endogenous cap-1 locus tagged with mScarlet-I at its N-terminus. (B) Schematic of the endogenous unc-94 locus tagged with mNeonGreenG at its C-terminus. (C) Cartoon of CAP-1 (magenta) and UNC-94 (green) performing their actin filament (blue) capping functions on the barbed (+) and pointed (−) ends, respectively. (D) Micrographs from a time-lapse movie depicting localization of mScarlet-I::CAP-1 and UNC-94::mNG over time from a ventral view. Yellow arrowheads point to Ea and Ep. (E and F) Plots depicting normalized fluorescence intensity of mScarlet-I::CAP-1 (E) and UNC-94::mNG (F) along the left–right axis of Ea (n = 10 embryos). Solid lines indicate the mean and shaded areas indicate the mean ± SD. Time represents minutes following the birth of the neighboring MSxx cells. Scale bar: 5 µm.
Figure 4.

Localization of barbed- and pointed-end capping proteins CAP-1 and UNC-94. (A) Schematic of the endogenous cap-1 locus tagged with mScarlet-I at its N-terminus. (B) Schematic of the endogenous unc-94 locus tagged with mNeonGreenG at its C-terminus. (C) Cartoon of CAP-1 (magenta) and UNC-94 (green) performing their actin filament (blue) capping functions on the barbed (+) and pointed (−) ends, respectively. (D) Micrographs from a time-lapse movie depicting localization of mScarlet-I::CAP-1 and UNC-94::mNG over time from a ventral view. Yellow arrowheads point to Ea and Ep. (E and F) Plots depicting normalized fluorescence intensity of mScarlet-I::CAP-1 (E) and UNC-94::mNG (F) along the left–right axis of Ea (n = 10 embryos). Solid lines indicate the mean and shaded areas indicate the mean ± SD. Time represents minutes following the birth of the neighboring MSxx cells. Scale bar: 5 µm.

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