Barbed-end actin filament proteins. (A) Schematic of the endogenous eps-8 locus tagged with mNG at its C-terminus. The start codons of the various isoforms are indicated with gray arrows. (B) Differential interference contrast (DIC, left) and fluorescence (right) micrographs of N2 control (blue) and EPS-8::mNG embryos (magenta) from a lateral view. (C) Schematic of the endogenous fli-1 locus tagged with mNG at its C-terminus. (D) DIC (top) and fluorescence (bottom) micrographs from a time-lapse movie depicting localization of FLI-1::mNG over time from a ventral view. (E) Schematic of the endogenous cap-1 locus tagged with either mScarlet-I or mNG at its N-terminus. (F) DIC (left) and fluorescence (right) micrographs depicting localization of mNG::CAP-1 from a ventral view. Ea and Ep are indicated with yellow dotted lines or arrowheads. Time represents minutes following the birth of the MSxx cells. Scale bars: 5 µm.