MRCK-1 exhibits slight enrichment at cell–cell borders and not at the center of the apex. (A) Micrographs from a time-lapse movie depicting dynamic localization of MRCK-1::YPET over time from a ventral view. MRCK-1 is covisualized with mScarlet-I::PH, which labels the plasma membrane. Yellow arrowheads point to Ea and Ep. (B) Plots depicting normalized fluorescence intensity of MRCK-1::YPET along the left–right axis of Ea (n = 12 embryos). Solid lines indicate the mean and shaded areas indicate the mean ± SD. Time represents minutes following the birth of the neighboring MSxx cells. Scale bar: 5 µm.
Figure 3.

MRCK-1 exhibits slight enrichment at cell–cell borders and not at the center of the apex. (A) Micrographs from a time-lapse movie depicting dynamic localization of MRCK-1::YPET over time from a ventral view. MRCK-1 is covisualized with mScarlet-I::PH, which labels the plasma membrane. Yellow arrowheads point to Ea and Ep. (B) Plots depicting normalized fluorescence intensity of MRCK-1::YPET along the left–right axis of Ea (n = 12 embryos). Solid lines indicate the mean and shaded areas indicate the mean ± SD. Time represents minutes following the birth of the neighboring MSxx cells. Scale bar: 5 µm.

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