Quantification of protein localization during apical constriction. (A–D) Plots depicting normalized intensity of mNG::NMY-2 (A, n = 11 embryos), MRCK-1::YPET (B, n = 12 embryos), CAP-1::mScarlet-I (C, n = 10 embryos), and mNG::UNC-94 (D, n = 10 embryos) across the anterior–posterior (A-P) or left–right (L-R) axes of Ea and Ep. Time represents minutes following the birth of the MSxx cells. Solid lines indicate the mean and shaded areas indicate the mean ± SD.