NMY-2 exhibits punctate localization distributed throughout the apical cortex during apical constriction. (A) Micrographs from a time-lapse movie depicting dynamic localization of mNG::NMY-2 over time from a ventral view. NMY-2 is covisualized with mScarlet-I::PH, which labels the plasma membrane. Yellow arrowheads point to Ea and Ep cells. (B) An enlarged micrograph from panel A to demonstrate how line scan (yellow line) measurements were collected. (C) Plots depicting fluorescence intensity of mNG::NMY-2, normalized to the mean intensity, along the left–right axis of Ea (n = 11 embryos). Solid lines indicate the mean, and shaded areas indicate the mean ± SD. Time represents minutes following the birth of the neighboring MSxx cells. Scale bars: 5 µm.
Figure 2.

NMY-2 exhibits punctate localization distributed throughout the apical cortex during apical constriction. (A) Micrographs from a time-lapse movie depicting dynamic localization of mNG::NMY-2 over time from a ventral view. NMY-2 is covisualized with mScarlet-I::PH, which labels the plasma membrane. Yellow arrowheads point to Ea and Ep cells. (B) An enlarged micrograph from panel A to demonstrate how line scan (yellow line) measurements were collected. (C) Plots depicting fluorescence intensity of mNG::NMY-2, normalized to the mean intensity, along the left–right axis of Ea (n = 11 embryos). Solid lines indicate the mean, and shaded areas indicate the mean ± SD. Time represents minutes following the birth of the neighboring MSxx cells. Scale bars: 5 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal