Full chimeras do not survive deletion of Mpc2 and/or Gls. Full chimeras were generated with bone marrow from WT (ROSA26 CreER^+/−), Mpc2 KO (Mpc2^fl/fl; ROSA26 CreER^+/−), Gls KO (Gls^fl/fl; ROSA26 CreER^+/−), or Gls Mpc2 DKO (Gls^fl/flMpc2^fl/fl; ROSA26 CreER^+/−) donors. Chimeras were provided tamoxifen chow for 14 d, and severe moribundity and survival were monitored over time. The single WT mouse that died was found in a flooded cage, suggesting an unrelated death. Data are pooled from two independent experiments. **, P < 0.01; ****, P < 0.0001 by log-rank test.