Deletion of Gls delays emergency myelopoiesis. (A) Schematic representation of cyclophosphamide experiment using Gls KO, Mpc2 KO, and Cre only control mixed bone marrow chimeras. Chimeric mice were injected with cyclophosphamide and then were bled on days 2 through 7 after treatment. (B) Peripheral blood cell counts of both CD45.1⁺ (WT) and CD45.2⁺ (WT, Gls KO, or Mpc2 KO) Ly6C^hi monocytes, neutrophils, B cells, and T cells from cyclophosphamide-treated chimeras. Values are normalized to pretreatment counts of each cell type. Data are pooled from two independent experiments. Mean values ± SEM are shown. *, P < 0.05; **, P < 0.01 by two-way ANOVA with post-hoc Tukey’s multiple comparisons test. (C) GMPs from WT or Gls KO chimeras were cultured with DMSO or 10 μM UK5099 in the presence of IL-3 and IL-6 for 7 d. On each day of the culture, cells were harvested and CD45.2 chimerism (left) and live cell counts (right) were obtained. Chimerism is normalized to the chimerism of the sorted GMPs. Cell counts are normalized to the total number of CD45.2⁺ cells plated on day 0. Data are pooled from two independent experiments with a total of 14 replicates from four mice per group. Mean values ± SEM are shown. **, P < 0.01; ***, P < 0.001 by two-way ANOVA with post-hoc Tukey’s multiple comparisons test.