Gating strategy and quantification of deletion of Cpt2 and Gls. (A) Representative flow cytometry gating strategy for splenic populations. (B) Representative flow cytometry gating strategy for bone marrow populations. (C) Quantitative PCR analysis of genomic Cpt2 deletion. CD45.2⁺ cells from the bone marrow or spleen were sorted for genomic DNA extraction. DNA quantification within loxP sites was quantified relative to WT cells and GAPDH. Mean values ± SEM are shown. Data are pooled from two independent experiments. ****, P < 0.0001 by Student’s two-tailed t test. (D) Representative flow cytometry gating strategy for platelets in the blood. (E) Quantitative PCR analysis of genomic Gls deletion. CD45.2⁺ GFP⁻ cells from the bone marrow or spleen were sorted for genomic DNA extraction. DNA quantification within loxP sites was quantified relative to WT cells and GAPDH. Mean values ± SEM are shown. Data are pooled from two independent experiments. ****, P < 0.0001 by Student’s two-tailed t test.