Analysis of single cell data for Cin8-degron data. (A) Median squared error of individual cells over a 35 min time course for Cin8-degron cells with no detachment events (red) and Cin8-degron cells with a detachment event (blue; P = 0.0029; Moods median test). (B) Median tension of individual cells over a 35 min time course for Cin8-degron cells with no detachment events (red) and Cin8-degron cells with a detachment event (blue; P = 0.062; Moods median test). (C) Fitted slope from linear regression of individual cells over a 35 min time course for Cin8-degron cells with no detachment events (red) and Cin8-degron cells with a detachment event (blue; P = 0.22; t test). (D) Fitted intercept from linear regression of individual cells over a 35 min time course for Cin8-degron cells with no detachment events (red) and Cin8-degron cells with a detachment event (blue; P = 0.22; t test). (E) Sum of squared error of individual time points from last 5 min of single cell traces, for Cin8-degron cells with no detachment events (red) and Cin8-degron cells with a detachment event (blue; P = 0.084; t test). (F) Standard deviation of tension for individual time points from last 5 min of single cell traces; Cin8-degron cells with no detachment events (red) and Cin8-degron cells with a detachment event (blue; P = 0.36; t test). (G) Mean tension, individual time points from last 5 min of single cell traces, for Cin8-degron cells with no detachment events (red) and Cin8-degron cells with a detachment event (blue; P = 0.077; t test).