Cin8-degron supporting information. (A) Method for arresting yeast cells in metaphase, and then degrading Cin8. (B) Theory to explain why degrading Cin8 leads to lower sister centromere stretching tension in yeast. (C) A comparison of single time point squared errors from Cin8-degron combined plots (Fig. 2 C) confirms increased tension variability in cells with detachment events as compared to those without detachment events (P = 0.09, t test, n = 34 time points in each data set). (D) A comparison of linear fit data for the tension history in Cin8-degron, benomyl-treated cells with (green) and without (magenta) a detachment event.