The effects of ionic strength on BKα channel modulation by the synthetic γ1 peptide mimic. (A) Representative current traces of BK channels formed by the BKα subunit under different intracellular conditions (peptide treatment and ionic strength) in response to depolarization of the membrane potential. (B) Voltage dependence of BKα channel activation with intracellular solutions of 1 M KMeSO₃ in the absence and presence of 20 µM γ1 peptide. (C) Voltage dependence of BKα channel activation with intracellular solutions of different ionic strength or osmolarity in the absence and presence of 20 µM γ1 peptide. The peptide was applied with a perfusion system. Currents were recorded in the virtual absence of [Ca²⁺]_i. Osmolarity was adjusted by sucrose for the low ionic strength solution of 50 mM KMeSO₃ and the high osmolarity solution containing 140 mM KMeSO₃. Error bars represent ± SEM.