Integrin α4 blocking traps migratory cells and inhibits the differentiation of stem-like CD8+T cells. Similar experimental setup to Fig. 5 A. (A) Representative FACS profiles of pre-gated Tcf-1+ (upper) and Tcf-1− (lower) P14 T cells to show the expression of CX3CR1 and CD69. (B) The percentage of CX3CR1+ cells in Tcf-1+ and Tcf-1− P14s are shown. (C) Representative FACS profiles of donor P14 T cells are shown. (D) The percentage of CD69+ in Tcf-1+ and Tcf-1− P14s are shown. (E–G) The percentage of IFN-γ+ (E), IL-2+ (F), and granzyme A+ (G) cells in Tcf-1+ and Tcf-1− P14s are shown. Each pair of symbols in B, D–F, and G represents the results from an individual recipient. Representative or pooled results from two independent experiments are shown (n = 4–5/each). *, P < 0.05; **, P < 0.01; ***, P < 0.001; and ****, P < 0.0001 by ordinary one-way ANOVA.
Figure 6.

Integrin α4 blocking traps migratory cells and inhibits the differentiation of stem-like CD8 + T cells. Similar experimental setup to Fig. 5 A. (A) Representative FACS profiles of pre-gated Tcf-1+ (upper) and Tcf-1− (lower) P14 T cells to show the expression of CX3CR1 and CD69. (B) The percentage of CX3CR1+ cells in Tcf-1+ and Tcf-1− P14s are shown. (C) Representative FACS profiles of donor P14 T cells are shown. (D) The percentage of CD69+ in Tcf-1+ and Tcf-1− P14s are shown. (E–G) The percentage of IFN-γ+ (E), IL-2+ (F), and granzyme A+ (G) cells in Tcf-1+ and Tcf-1− P14s are shown. Each pair of symbols in B, D–F, and G represents the results from an individual recipient. Representative or pooled results from two independent experiments are shown (n = 4–5/each). *, P < 0.05; **, P < 0.01; ***, P < 0.001; and ****, P < 0.0001 by ordinary one-way ANOVA.

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