TGF-β controls the lymphoid tissue retention of Tcf-1 ⁺ CD8 ⁺ T cell subset. Same experimental setup as in Fig. 1. (A) Day 7 and day 12 after infection, representative FACS profiles of pre-gated donor WT P14 T cells are shown (two independent repeats, n = 4–5/each). (B) The percentage of Tcf-1⁺Slamf6⁺ subset in WT P14 T cells from peripheral blood is shown. (C) Day 12 after infection, the ratio of Tgfbr2^−/− Tcf-1⁺ over control Tcf-1⁺ P14 T cells from different tissues is shown. (D) Representative FACS profiles of pre-gated Tcf-1⁺ P14 T cells are shown (two independent repeats). (E) The percentage of β1⁺β7⁺ cells in each Tcf-1⁺ subset is shown. Each symbol in B and each group of connected symbols in C and E represents the results from an individual recipient mouse. Pooled results from five (B, total n = 5–10) or two (C and E, total n = 10) independent experiments are shown. *, P < 0.05; ***, P < 0.001; and ****, P < 0.0001 by ordinary one-way ANOVA.