Absolute protein abundance quantification of HaloTagged autophagy factors. (A–D) Representative fluorescence gels for the absolute quantification of the autophagy proteins. (E) Standard curve for cell number using stain-free gels (left) and florescent HaloTag protein (right), demonstrating an excellent correlation between intensity and gel loadings. (F) Representative fluorescence gel of HaloTagged proteins in the absence or presence of TEV protease. (G) Ratio of HaloTag fluorescence in the absence and presence of TEV protease (B; N = 3, mean ± SD). Source data are available for this figure: SourceData FS3.
Figure S3.

Absolute protein abundance quantification of HaloTagged autophagy factors. (A–D) Representative fluorescence gels for the absolute quantification of the autophagy proteins. (E) Standard curve for cell number using stain-free gels (left) and florescent HaloTag protein (right), demonstrating an excellent correlation between intensity and gel loadings. (F) Representative fluorescence gel of HaloTagged proteins in the absence or presence of TEV protease. (G) Ratio of HaloTag fluorescence in the absence and presence of TEV protease (B; N = 3, mean ± SD). Source data are available for this figure: SourceData FS3.

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