Figure S3.
Mapping of Golgi markers with STED microscopy in nocodazole-treated cells. (A–G) HeLa cells were treated with nocodazole (33 µM) for 3 h, fixed and immunostained as indicated in the figure. Secondary antibodies labeled either with ATTO647N or AlexaFluor594 were used to be able to perform dual-color STED experiments. (H and I) Scatter dot plots with mean and SD represent the quantification of the distances from the edge of the cis-labeled (H, GM130) or trans-labeled (I, Golgin97) cisternae to the center of COPI and clathrin vesicles measured in the nocodazole-treated fixed cells as described in Materials and methods. GM130: clathrin n = 35; COPI n = 38 (H). Golgin97: clathrin n = 52; COPI n = 56 (I). n = number of ministacks analyzed from at least three independent experiments. All images were background subtracted and smoothed with a Gaussian filter as described in Materials and methods. Scale bars are 1 µm.

Mapping of Golgi markers with STED microscopy in nocodazole-treated cells. (A–G) HeLa cells were treated with nocodazole (33 µM) for 3 h, fixed and immunostained as indicated in the figure. Secondary antibodies labeled either with ATTO647N or AlexaFluor594 were used to be able to perform dual-color STED experiments. (H and I) Scatter dot plots with mean and SD represent the quantification of the distances from the edge of the cis-labeled (H, GM130) or trans-labeled (I, Golgin97) cisternae to the center of COPI and clathrin vesicles measured in the nocodazole-treated fixed cells as described in Materials and methods. GM130: clathrin n = 35; COPI n = 38 (H). Golgin97: clathrin n = 52; COPI n = 56 (I). n = number of ministacks analyzed from at least three independent experiments. All images were background subtracted and smoothed with a Gaussian filter as described in Materials and methods. Scale bars are 1 µm.

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