The Hck ^−/− Fgr ^−/− Lyn ^−/− mutation attenuates inflammation in the motheaten viable model. (A) Representative images of the hind paws of WT, Ptpn6^me-v/me-v, and Ptpn6^me-v/me-vHck^−/−Fgr^−/−Lyn^−/− mice. (B and C) H&E-stained sections (B; upper panels: 10× magnification, scale bar, 100 µm; lower panels: 40× magnification, scale bar, 25 µm) and weight (C) of lungs from Ptpn6^me-v/me-v and Ptpn6^me-v/me-vHck^−/−Fgr^−/−Lyn^−/− mice. (D) Survival of the mice of the indicated genotypes. (E–G) neutrophils isolated from mice of the indicated genotypes were stimulated on fibrinogen surface (E and G) or by using 1 nM PMA (F), and superoxide release (E and F) or the percentage of cellular adhesion (G) was determined. Panels A and B show representative images from four independent experiments. Bar graphs show mean and SEM of 15–19 mice per genotype (C) and mean and SEM from three independent experiments (G). Survival curves show the data of 10–29 animals per genotype. Kinetic curves on panels E and F show representative experiments performed in triplicate (mean and SEM) from four independent experiments. Cells were prepared from three to four mice per genotype and pooled for each experiment. One-way ANOVA (C and G), log-rank test (D); n.s., not significant; ***, P < 0.001. See the text for actual P values.