Dasatinib inhibits MSU crystal–induced proinflammatory functions of human neutrophils. Human neutrophils were pretreated with 100 nM or the indicated concentrations of dasatinib or 10 μM cytochalasin D, followed by stimulation with 1 mg/ml MSU crystals. (A) Luminometric measurement of reactive oxygen production. (B and C) LTB4 release determined from cell-free supernatants after 18 h incubation with the crystals of unprimed (B) or TNF-primed (10 ng/ml TNF; C) neutrophils. (D) Analysis of intracellular p38/ERK phosphorylation. (E–H) Phagocytosis of the MSU crystals assessed by flow cytometry (E and G) or phase contrast microscopy (F and H; scale bar: 20 µm). Panels D, E, and F show representative images. Bar graph shows mean and SEM from four to six (A), two to three (B and C), and three (G and H) independent experiments. RLU, relative luminescence unit; AUC, area under the curve. Two-way ANOVA inhibitor × stimulus interaction analysis (A–C and G) and one-way ANOVA (H); n.s., not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001. See the text for actual P values. Source data are available for this figure: SourceData F5.