Analysis of the inflammatory microenvironment. (A–L) WT or Hck^−/−Fgr^−/−Lyn^−/− mice were subjected to gouty arthritis as described in Fig. 2. (A–D) In vivo MPO (A and B) and NADPH-oxidase (C and D) activity was determined by chemiluminescence imaging after i.p. injection of luminol or lucigenin, respectively. Color-coded photon flux intensity is superposed on the grayscale photo of the mice after 24 h of MSU injection (A and C) and quantified in defined regions of interest at the indicated time points (B and D). (E–G) The hind paws were flushed after 24 h of MSU injection and the number of neutrophils (E) or monocytes/macrophages (F and G) was determined by flow cytometry. (H–L) The cell-free supernatants of the tissue infiltrates were probed using a commercial cytokine array (H and I) or analyzed by ELISA for the indicated pro-inflammatory mediators (J–L). Panels A, C, and H show representative images. Bar graphs show mean and SEM from 6–12 (B and D), 8–20 (E), 3 (F and G), 2 (I), and 13–27 (J–L) mice per genotype from 2 (B and D), 8 (E), 1 (F and G), 2 (I), and 4–7 (J–L) independent experiments. Two-way ANOVA genotype × stimulus interaction analysis; *, P < 0.05; **, P < 0.01; ***, P < 0.001. See the text for actual P values. Source data are available for this figure: SourceData F3.