Myeloid Src-family kinases mediate MSU crystal–induced arthritis in vivo. (A–G) WT or Hck^−/−Fgr^−/−Lyn^−/− mice or various single and double mutants (D and E) were subjected to gouty arthritis by injecting the right hind paws of the mice with 1 mg MSU crystal suspended in 50 μl PBS, along with injecting left hind paws with PBS alone as controls. MSU crystal–induced inflammation was followed by photographing on day 1 (A; scale bar: 10 mm), measuring the paw volume by plethysmometry (B–E), and examining hypernociception (F and G). Panel A shows a representative experiment. Quantitative curves and bar graphs show mean and SEM from 11 to 13 (B), 32–33 (C), 5–13 (D and E; except for a single Hck^−/−Lyn^−/− mouse), and 11–16 (F and G) mice per genotype from 3 (B), 5 (C), 2–3 (D and E), and 2 (F and G) independent experiments. Two-way ANOVA genotype × stimulus (A–E) or genotype × time (F and G) interaction analysis; *, P < 0.05; **, P < 0.01; ***, P < 0.001. See the text for actual P values.