MSU crystal–induced macrophage responses. (A–D) WT or Hck−/−Fgr−/−Lyn−/− bone marrow–derived macrophages were stimulated with 1 mg/ml MSU crystals without (A–D) or with (B–D) overnight priming of the cells with 1 μg/ml ultrapure LPS. (A and B) Luminometric measurement of reactive oxygen production. (C and D) IL-1β (C) and MIP-2 (D) levels determined from cell-free supernatants after incubation for 24 h. Kinetic curves (A) show mean and SD of a representative experiment. Bar graph shows mean and SEM from three (B) or four to five (C and D) independent experiments. RLU, relative luminescence unit. Two-way ANOVA genotype × stimulus interaction analysis; n.s., not significant; **, P < 0.01; ***, P < 0.001. See the text for actual P values.
Figure S2.

MSU crystal–induced macrophage responses. (A–D) WT or Hck−/−Fgr−/−Lyn−/− bone marrow–derived macrophages were stimulated with 1 mg/ml MSU crystals without (A–D) or with (B–D) overnight priming of the cells with 1 μg/ml ultrapure LPS. (A and B) Luminometric measurement of reactive oxygen production. (C and D) IL-1β (C) and MIP-2 (D) levels determined from cell-free supernatants after incubation for 24 h. Kinetic curves (A) show mean and SD of a representative experiment. Bar graph shows mean and SEM from three (B) or four to five (C and D) independent experiments. RLU, relative luminescence unit. Two-way ANOVA genotype × stimulus interaction analysis; n.s., not significant; **, P < 0.01; ***, P < 0.001. See the text for actual P values.

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