Analysis of PMA-induced superoxide production. (A and B) WT or Hck^−/−Fgr^−/−Lyn^−/− neutrophils (A) and human neutrophils treated with vehicle or 100 nM dasatinib (B) were stimulated with 100 nM PMA, followed by a spectrophotometric analysis of superoxide release. Kinetic curves (A and B) show mean and SEM of five (A) or three (B) independent experiments. Two-way ANOVA genotype × stimulus (A) or inhibitor × stimulus (B) interaction analysis; n.s., not significant. See the text for actual P values.