HSCs responded to LPS and pIpC stimulation. (A) Expression levels of Tlr4 protein in LT-HSC and LSK shown in histograms and the relative expression ratio of Tlr4 to the isotype control. (B) Expression levels of Tlr3 protein in LT-HSC and LSK and the relative expression ratio of Tlr3 to the isotype control. (C) Purified WT HSCs responding to LPS and/or pIpC stimulation in an in vitro condition to activate transcription of canonical NF-κB target genes and IFN-stimulated genes such as Junb and Irf7 examined by RamDA-seq and q-RT-PCR. Bars show the mean ± SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001. P values were calculated by Student’s t test. Data are representative of two independent experiments.