Identification of EBRs within the Ft ICD. (A) Identification of EBR2 in the Ft ICD. HEK293 cell expression and IP of indicated FLAG-tagged Ft^ΔECD constructs in the presence of Ex^FERM. (B) Ft conserved C and D regions affect interaction with Ex. HEK293 cell expression and IP of indicated FLAG-tagged Ft^ΔECD constructs in the presence of Ex^FERM. The expression and presence of proteins was analyzed by immunoblotting with the indicated antibodies. Ft presents as multiple bands due to proteolytic processing (Feng and Irvine, 2009; Sopko et al., 2009). (C) Graphical scheme highlighting the Ft constructs used in the figure. In addition, the transmembrane domain (TM), EBR1, EBR2, and established conserved and function domains of the Ft ICD are depicted. In binding column: “++” denotes constructs that interact strongly to Ex^FERM, “+” denotes weak interaction with Ex^FERM, and “−” denotes no interaction with Ex^FERM. WB, Western blot. Source data are available for this figure: SourceData FS3.