CSLD trajectories do not align with cortical microtubules. (A) Time-lapse VAEM imaging of protonemata expressing mScarlet-CSLD6 (magenta in the merged image) and mEGFP-tubulin (green in the merged image) demonstrate that the majority of CSLD6 particles do not associate with cortical microtubules. Scale bar for all images in A, 5 µm. Also see Video 7. (B and C) Lines on the image were used to produce the kymographs shown in C. Scale bar in B, 5 µm. (C) Kymographs demonstrate that mScarlet CSLD6 (magenta) moves in linear trajectories that are not associated with microtubules (green). Horizontal scale bar in C, 2 µm. Vertical scale bar in C, 1 min.