Localization of endogenously tagged CSLD1-8 with mScarlet in a moss line stably expressing mEGFP-tubulin. (A) In protonemata, CLSDs localized to punctate structures and are enriched on the plasma membrane near the cell apex. (B) During cell division, CSLDs accumulate on the expanding cell plate. Scale bar, 5 µm. All images are single focal planes acquired on a laser scanning confocal microscope.